FIREScript® RT cDNA synthesis KIT
FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C to 60°C. FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).
Reagents FIREScript® Reverse Transcriptase (200 U/μl) RiboGrip RNase inhibitor (40 U/μl) 10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT) Oligo (dT) Primer (100 μM) Random Primers (100 μM) dNTP MIX (20 mM of each) Water, nuclease free